Coding

Part:BBa_K533006

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)

Multi-Proline-mCherry

mCherry protein with a his tag for purification and a proline rich peptide for association with SH3 domain.

Usage and Biology

After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression)

Thuexp cherryexp.png

From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 109
    Illegal BamHI site found at 908
    Illegal XhoI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None